Journal: BMC Cancer

Article Title: Recurrence in oral and pharyngeal cancer is associated with quantitative MGMT promoter methylation

doi: 10.1186/1471-2407-9-354

Figure Lengend Snippet: Degree of MGMT promoter methylation . The degree of methylation at the MGMT promoter was determined by pyrosequencing (Pyromark MGMT ID system). Shown are the plots of (A) overall patient survival time (months) and (B) disease-free survival time (months), according to the degree of MGMT methylation (no detectable methylation or a level of methylation either greater than or less than 6.9%).

Article Snippet: The treated DNA was amplified by PCR with MGMT-specific primers and twenty microliters of the amplicon was then subjected to pyrosequencing using the Biotage PyroMark Q24 System.

Techniques: Methylation

Journal: PLoS ONE

Article Title: Validation of SNP Allele Frequencies Determined by Pooled Next-Generation Sequencing in Natural Populations of a Non-Model Plant Species

doi: 10.1371/journal.pone.0080422

Figure Lengend Snippet: Comparison between major allele frequencies calculated from individual genotyping using pyrosequencing (PyroMark) and based on pooled population samples (Pool-Seq) determined with Illumina. In , allele frequencies from the Pool-Seq were calculated from reads mapped to the publicly available Arabidopsis thaliana genome. In , the reads were mapped to our own de novo draft genome of A. halleri . Shown are the results of SNPs from six genes for all three populations studied. The dashed line represents the expected 1:1 proportion. Open circles represent comparisons including incomplete date from the pyrosequencing, filled circles refer to comparisons with complete data. Note that some data points are overlapping.

Article Snippet: The dispensation order of the four nucleotides was determined automatically by the PyroMark ID software (Biotage), and genotyping was performed using default settings.

Techniques: Comparison